Cas9 Nuclease is an RNA-guided sequence-specific double-stranded DNA endonuclease. The guide RNA recognizes the target site by being complementary to the target sequence and brings Cas9 Nuclease to the target DNA. Cas9 Nuclease has two cleavage active centers, which respectively cut the two strands of target DNA to produce DNA double-strand breaks. The cutting site is three bases away from NGGPAM in the targeted region. This product is a high-purity Cas9 active protein expressed and purified in E. coli after the Cas9 gene of Streptococcus pyogenes has been cloned.   High purity and high activity Cas9 protein; Convenient for in vitro sgRNA efficiency verification   Store at -20°C