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Molecular Research

NGS Library Prep kits

Molecular Research

Drug Discovery

Molecular Research

185.00
Article number:
Q222-CN-00
185.00
Article number:
Q222-CN-00
Number of views:
1000
Keywords:
1200.00
Article number:
HF001-1 
HiscriptHighFidelityOneStepRT-PCRKit一步法高效RT-PCR试剂盒逆转录和PCR在同一管内进行检测灵敏度可达0.1pg总RNA可扩增超过10kb的长片段图1A.以Hela细胞总RNA为模板,用Hiscript® HighFidelityOneStepRT-PCRKit (HF001)扩增555bpGAPDH片段。检测灵敏度可达到0.1pg。B.以猪蓝耳病毒PRRSVRNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增660bp片段。检测灵敏度可达到0.2TCLD50。C.以1μgHela细胞总RNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增不同长度的片段。可成功扩增长达12.1kb的UTRN片段。本试剂盒专为以RNA为模板(如RNA病毒)的终点法PCR检测而设计。使用基因特异引物(GSP),逆转录和PCR反应在一管内完成,不需要额外的开管/移液操作,大大提高了检测通量,并降低了污染的风险。整合HiScript®IIReverseTranscriptase以及ChampagneTaqTMDNAPolymerase的优越性能,配合经过优化的缓冲体系,Hiscript®HighFidelityOneStepRT-PCRKit(HF001) 可扩增片段长达10kb以上。试剂盒以便捷的MasterMix形式提供。2×OneStepMix包含优化的缓冲体系和dNTPs;OneStepEnzymeMix包含比例优化的HiScript®IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase,操作简单快捷。组分HF001-0150rxn(50ul/rxn)RNasefreeddH2O1ml×22×OneStepMixa625μl×2OneStepEnzymeMixb125μl10×Loadingbuffer1.25mla.包含dNTP和缓冲体系b.包含HiScript® IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase-30~-15℃保存。
1200.00
Article number:
HF001-1 
Number of views:
4965
Keywords:
fidelity
one
step
hiscript
片段
rt-pcr
扩增
rna
kit
检测
HF001-01
HF001
hf001
9999.00
Article number:
DC112-01/02
This kit is mainly suitable for extracting genomic DNA from a variety of fresh or frozen anticoagulant blood, cells, animal tissues and bacterial samples. The kit uses silica gel membrane purification technology, no need to use toxic reagents such as phenol, chloroform, and time-consuming alcohol precipitation during the extraction process, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.   High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity, and complete fragments. Wide range of samples: suitable for direct extraction of DNA from a variety of fresh or frozen anticoagulated blood, cells, animal tissues and bacterial samples. Simple and fast: Animal tissue provides a 30min quick extraction plan.     Proteinase K is stored at 2-8°C, and other components are stored at room temperature (15-25°C).
9999.00
Article number:
DC112-01/02
Number of views:
1458
Keywords:
bacteria
and
the
of
dna
extraction
animal
high
for
extracted
9999.00
Article number:
R504-01
RNase and nucleic acid scavenger, a non-toxic spray reagent, used to remove RNase contamination on the surface of instruments, equipment, and experimental desktops. At the same time, it can establish an RNA-related experimental operating environment for nucleic acid templates without affecting the stability of RNA Sex.   High removal efficiency: can completely remove nucleic acid templates such as RNase and DNA/RNA in the experimental environment  Safe, non-toxic, easy to use: liquid, spray and use No damage to equipment: non-corrosive, can be sprayed directly on the surface of the instrument   Store at room temperature (15 ~ 25℃)    
9999.00
Article number:
R504-01
Number of views:
1286
Keywords:
rna
the
and
experimental
acid
rnase
can
of
nucleic
to
9999.00
Article number:
QV110 -01/02
Animal Detection U+ Probe Master Mix is ​​a special premix suitable for the detection of African swine fever virus (ASFV) by the probe method. It only needs to add additional primers, probes and templates, which is easy to use. This premix uses an upgraded hot-start Taq enzyme and a carefully optimized Buffer to improve the detection sensitivity of low-concentration templates. This product introduces the dUTP/UDG anti-pollution system, which can function at room temperature to remove the pollution in the system and ensure the accuracy of the results. At the same time, it is compatible with fast programs, reducing detection time.   Excellent amplification sensitivity: The upgraded hot-start Taq DNA polymerase with a carefully optimized buffer system improves the detection sensitivity of low-concentration templates; dUTP/UDG anti-pollution system: Introduce dUTP/Heat-labile UDG anti-pollution system to efficiently degrade aerosol pollution of products, reduce false positives, and ensure that the results are true and reliable; Support fast program: Compatible with fast program, the result detection can be completed within 40 minutes; Excellent storage stability: The reagent has stable performance when stored at 37°C for 9 days, repeated freezing and thawing 30 times, and transported for 4 days.    Store at -30~-15°C
9999.00
Article number:
QV110 -01/02
Number of views:
1583
Keywords:
detection
the
and
system
to
of
at
is
sensitivity
55.00
Article number:
P033-01/P034-01/P035-01/P036-01
55.00
Article number:
P033-01/P034-01/P035-01/P036-01
Number of views:
1569
Keywords:
56.00
Article number:
P525-01/02/03
Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).   Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples Simple operation: Dye premix, minimize experimental steps    -Store at 20°C  
56.00
Article number:
P525-01/02/03
Number of views:
4911
Keywords:
dye
and
the
of
can
amplification
to
phanta
max
dna
9999.00
Article number:
R701-01/02
RNA-easy Isolation Reagent is widely used to extract Total RNA and Small RNA from samples of various animal tissues, plant materials, cultured cells and microorganisms. Compared with the traditional Trizol extraction method, this product is simple to use, does not require the use of chloroform for stratification, and the whole process can be performed at room temperature; in addition, this product effectively inhibits the activity of RNase (RNase) while simultaneously removing protein and DNA. , Polysaccharides and other impurities precipitate to the bottom of the tube to achieve single-phase extraction and effectively ensure the integrity and purity of RNA. The entire extraction process can be completed within 50 minutes using this product. The extracted RNA can be directly used in various molecular biology such as cDNA cloning, qRT-PCR detection, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing, etc. experiment.   Broad platform compatibility: The addition of universal ROX to miRNA Universal SYBR® qPCR Master Mix enables mix to be compatible with qPCR instruments on different platforms without the need to adjust ROX concentration.   Store at 2 ~ 8℃.     
9999.00
Article number:
R701-01/02
Number of views:
4294
Keywords:
reagent
the
to
and
of
be
in
extraction
rna
this
9999.00
Article number:
P073-01/02/03
RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.   Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.   Store at 2~8℃.
9999.00
Article number:
P073-01/02/03
Number of views:
2708
Keywords:
roomtemp
blood
the
lysis
and
for
can
dna
method
as
9999.00
Article number:
P115-01/02
This product contains 3G Taq DNA Polymerase, dNTP, visual red dye and optimized buffer system. It is easy to use, reduces pollution, and improves detection throughput and results reproducibility. 3G Taq DNA Polymerase has higher inhibitor tolerance and amplification efficiency than wild-type Taq DNA Polymerase. This product has been added with a visual red dye, which can be directly used for polyacrylamide gel electrophoresis and agarose gel electrophoresis after the reaction, which is easy to operate.   Bright: Contains visual red dye, the color is eye-catching after reaction, suitable for high-throughput detection; Convenience: The product is 2 × ready-to-use Mix, which reduces pipetting operations and is easy to use; Excellent amplification performance: 3G Taq DNA Polymerase has higher inhibitor tolerance and amplification efficiency, which improves the success rate of experiments.    Store at -30 ~ -15℃
9999.00
Article number:
P115-01/02
Number of views:
2977
Keywords:
red
and
is
dna
which
taq
the
3g
visual
143.00
Article number:
C311
5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.   Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.    Store at -30 ~ -15°C
143.00
Article number:
C311
Number of views:
3859
Keywords:
5min
and
ends
the
ligation
compatible
dna
connection
can
reaction
99.00
Article number:
DC111-01/02
This kit is mainly suitable for quickly and easily extracting genomic DNA from 0.1-1 ml fresh and frozen anticoagulated whole blood samples. The kit uses silica gel membrane purification technology, and no toxic reagents such as phenol and chloroform are required during the extraction process. There is no need for time-consuming alcohol precipitation, and the maximum removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as enzyme digestion, PCR, Southern blotting, etc.   High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility Except for Proteinase K, the other components of the kit should be stored at 15-25°C; Proteinase K can be stored at 15-25℃ for three months, long-term storage needs to be placed at 2-8℃.  
99.00
Article number:
DC111-01/02
Number of views:
2928
Keywords:
mini
and
the
for
be
of
at
kit
high
dna
9999.00
Article number:
S103-01
This kit uses a DNA polymerase with extremely high fidelity and an optimized reaction system to quickly amplify plasmids from bacterial liquid or colonies to obtain DNA that can be used for sequencing reactions. Using this kit to prepare plasmid DNA for sequencing reactions can save a lot of shaking time and cost, simplify the sample preparation process, and accelerate the speed from sample receipt to result output. Under normal circumstances, each reaction can produce 1.5-2.5 µg of plasmid DNA. The product DNA can be used directly in the sequencing reaction.   Rapid amplification of plasmids, directly used in sequencing reactions; Save shaking time and cost, simplify sample preparation process    Store at -20℃, except for Enzyme Mix v2, the rest can be stored at 4℃ for 2 weeks after defrosting
9999.00
Article number:
S103-01
Number of views:
2151
Keywords:
amplification
can
the
sequencing
dna
for
and
to
used
be
242.00
Article number:
R323-01
HiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the optimal buffer optimized for reverse transcription further improve the efficiency of cDNA synthesis and are suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis.    Easy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes; Extensive template compatibility: Compatible with RNA templates of different species and poor integrity; Super impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.; Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.   Store at -30~-15°C
242.00
Article number:
R323-01
Number of views:
13662
Keywords:
gdna
the
reverse
transcription
and
for
hiscript
of
supermix
iii
174.00
Article number:
R312-01/02
HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.   Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns    Store at -30~-15°C (restore 10*RT Mix to room temperature and shake well for use)
174.00
Article number:
R312-01/02
Number of views:
8935
Keywords:
synthesis
the
reverse
primers
for
and
of
to
can
transcription
144.00
Article number:
R302-01
HiScript® III Reverse Transcriptase is an upgraded version of HiScript® II Reverse Transcriptase, which can perform highly efficient reverse transcription reactions at 37°C. HiScript® III Reverse Transcriptase still retains the thermal stability of the second-generation product HiScript® II Reverse Transcriptase. For RNA with complex secondary structure, the reverse transcription temperature can be increased To 50~55℃, avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and can effectively synthesize high-quality cDNA. In addition, this product still has superior continuous synthesis ability and super Strong impurity tolerance.    Extensive template compatibility: compatible with various templates such as animals, plants, viruses, etc.; Super impurity tolerance: It has super tolerance to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid); Excellent reverse transcription efficiency: reverse transcription efficiency is higher than that of second-generation products    Store at -30~-15°C    
144.00
Article number:
R302-01
Number of views:
3986
Keywords:
hiscript
reverse
transcription
of
the
can
transcriptase
super
impurity
127.00
Article number:
DC105
This product is suitable for separation and purification of genomic DNA from formalin-fixed and paraffin-embedded tissues, and overcomes the inhibitory effect caused by formalin cross-linking. The dewaxing solution used in this kit is safer than xylene; and high-quality DNA can be obtained through a centrifugal adsorption column that specifically binds DNA and a unique buffer system. The entire extraction process only takes 20 minutes (except the digestion time). The extracted genome has good integrity, high purity, stable and reliable quality, and can be used in a variety of downstream applications, such as PCR, qPCR, library construction and other experiments.   safe and non-toxic; fast; Efficient high production   The components of this product except Proteinase K can be stored at 15 ~ 25℃ for 12 months. If you need to store it for a longer time, store it at 2 ~ 8℃. If the solution precipitates under storage conditions of 2 ~ 8℃, the solution in the kit should be placed at room temperature for a period of time before use, and turned upside down until the precipitate is completely dissolved; if necessary, preheat it in a 37℃ water bath for 10 minutes to dissolve the precipitate.
127.00
Article number:
DC105
Number of views:
2408
Keywords:
dna
the
and
for
of
in
be
if
is
152.00
Article number:
Q511-02/03
This product is a special reagent for qPCR using the SYBR® Green I chimeric fluorescence method. It uses a chemically modified hot-start DNA polymerase AceTaq® DNA Polymerase, with the optimal buffer optimized for qPCR, which can effectively inhibit non-specific amplification. Thus, the amplification efficiency is significantly improved, and it is suitable for high-sensitivity qPCR reactions. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high. In addition, the product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.   Universal on all platforms: Special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides perfect amplification specificity; Optimized reaction body: patented buffer formula, which minimizes non-specific amplification and primer dimerization, without repeated optimization of conditions; Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines    Store at -20℃ and avoid light
152.00
Article number:
Q511-02/03
Number of views:
8446
Keywords:
sybr
the
and
is
for
qpcr
to
amplification
of
rox
203.00
Article number:
P621-01
The Single Cell Squence Specific Amplification Kit is based on a one-step RT-PCR amplification method, which is used to realize the amplification of the transcriptome in single cells or trace amounts of total RNA, so that you can uncover the expression levels of different genes between individual cells. This kit realizes that RNA extraction and purification, reverse transcription and PCR reactions are completed in the same tube, no additional operations are required, and it has the advantages of saving time, reducing experimental errors, reducing pollution, and improving sensitivity. This kit is also suitable for one-step expansion of 2-1000 cells, and the number of cycles of one-step expansion should be reduced according to the number of cells.   Suitable for 1-10,000 cells; One-step RT-PCR pre-amplification; Up to 500 target genes can be amplified; The amplified product is suitable for analysis by any real-time fluorescent quantitative PCR instrument; High sensitivity, low cost    Store at -20℃
203.00
Article number:
P621-01
Number of views:
3330
Keywords:
single
the
of
and
is
one-step
amplification
to
for
kit
152.00
Article number:
Q441-02/03
This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.   Reduce pipetting errors: By providing reagents of different colors, using the color change effect during pipetting, tracking the pipetting process and reducing pipetting errors; Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification performance: single-digit copy gene expression can be detected, and the target gene location is accurate and reliable; Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines   2×ChamQ SYBR Color qPCR Master Mix (High ROX Premixed): long-term storage at -20°C and protected from light, after thawing, stable storage at 4°C and protected from light for 6 months; 10×Dilution Buffer: storage at -20°C
152.00
Article number:
Q441-02/03
Number of views:
2728
Keywords:
sybr
the
and
amplification
is
pipetting
of
dna
at
polymerase
152.00
Article number:
Q431-02/03
This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.   Reduce pipetting errors: By providing reagents of different colors, using the color change effect during pipetting, tracking the pipetting process and reducing pipetting errors; Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification performance: single-digit copy gene expression can be detected, and the target gene location is accurate and reliable; Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines   2×ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed): long-term storage at -20°C and protected from light, after thawing, stable storage at 4°C and protected from light for 6 months; 10×Dilution Buffer: storage at -20°C
152.00
Article number:
Q431-02/03
Number of views:
2908
Keywords:
sybr
the
and
amplification
is
pipetting
of
dna
at
polymerase
152.00
Article number:
Q421-02/03
ChamQTMSYBRColorqPCRMasterMix(WithoutROX)模板示踪型染料法定量PCR检测试剂盒减少移液错误:通过提供不同颜色的试剂,利用移液过程中的变色效应,追踪移液过程,减少移液错误。扩增高度特异:新型抗体法热启动酶ChampagneTaqTM  DNAPolymerase,配以优化的Buffer和专利特异性促进因子ExactorTM,有效避免引物二聚体和非特异性扩增的产
152.00
Article number:
Q421-02/03
Number of views:
2122
Keywords:
ChamQTM SYBR Color qPCR Master Mix( Without ROX )
Q421-02/03
152.00
Article number:
Q411-02/03
This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.   Reduce pipetting errors: By providing reagents of different colors, using the color change effect during pipetting, tracking the pipetting process and reducing pipetting errors; Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification performance: single-digit copy gene expression can be detected, and the target gene location is accurate and reliable; Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines   2×ChamQ SYBR Color qPCR Master Mix: long-term storage at -20°C and protected from light. After thawing, it can be stored stably at 4°C and protected from light for 6 months; 10×Dilution Buffer: stored at -20°C; 50×ROX Reference Dye1/2: Store at -20℃
152.00
Article number:
Q411-02/03
Number of views:
5565
Keywords:
sybr
the
and
amplification
is
at
pipetting
of
dna
20
152.00
Article number:
Q711-02/03
This product is a special premix for qPCR reaction using SYBR® Green I chimeric fluorescence method. The core component ChampagneTM Taq DNA Polymerase is a new type of antibody-based hot-start DNA polymerase, which has many advantages such as strong specificity and high detection sensitivity. It is equipped with the most suitable buffer optimized for qPCR and the patented specific promoter ExactorTM, which is very suitable For high specificity and high sensitivity qPCR reaction. This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.   Wide platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments; Ultra-high amplification specificity: the use of a new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification sensitivity: The master mix has an excellent linear relationship in a wide template range, and can detect single-digit copies of the template to be tested; Excellent program compatibility: Standard programs and fast programs can be used to quantify the premix, which perfectly solves program compatibility.     Store at -20°C in the dark; Master Mix can be stored stably at 4°C in the dark for 6 months after thawing
152.00
Article number:
Q711-02/03
Number of views:
13904
Keywords:
sybr
the
and
is
to
for
of
qpcr
which
rox
222.00
Article number:
Q811-02/03
This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.   Reduce pipetting errors: By providing reagents of different colors, the color change effect during pipetting is used to track the pipetting process and reduce pipetting errors. Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patented specific promotion factor ExactorTM, effectively avoids primer dimers and non-specific amplification. Excellent amplification performance: single-digit copy gene expression can be detected, and target gene positioning is accurate and reliable. Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines   Store at -30~-15°C away from light
222.00
Article number:
Q811-02/03
Number of views:
3971
Keywords:
geno-snp
the
and
is
amplification
pipetting
dna
polymerase
of
addition
17.00
Article number:
DE103
Lysozyme (lysozyme) is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. It mainly destroys the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall. The cell wall insoluble mucopolysaccharide is decomposed into soluble glycopeptides, causing the cell wall to rupture and the bacteria to dissolve. Mainly used for the digestion of the cell wall of gram-positive bacteria during the preparation of DNA and RNA.   High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility   Except for Proteinase K, the other components of the kit should be stored at 15-25°C; Proteinase K can be stored at 15-25℃ for three months, long-term storage needs to be placed at 2-8℃.  
17.00
Article number:
DE103
Number of views:
2196
Keywords:
lysozyme
the
of
cell
at
be
for
and
to
wall
171.00
Article number:
RC401
This kit can quickly extract the total RNA of multiple samples from plant tissues, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, pine needles, bananas, potato tubers, grapes, rose flowers, buckwheat, etc.). The kit is based on silica gel column purification technology. There is no need to use toxic phenol-chloroform extraction during the extraction process. The whole process only takes 30 minutes. The FastPure gDNA-Filter Column II in the kit can effectively filter impurities and specifically adsorb and remove genomic DNA. FastPure RNA Column Ⅳ can efficiently bind RNA, and is equipped with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used for RT-PCR, Real-time PCR, chip analysis, Northern Blot And other downstream experiments.   Safety; fast; Wide compatibility; High purity; Less genome residue   Store at room temperature (15-25℃)
171.00
Article number:
RC401
Number of views:
4178
Keywords:
polyphenolics
the
and
is
rna
can
column
kit
of
tissues
116.00
Article number:
DC202
This kit is suitable for extracting 150-300 ml of overnight cultured bacteria, using an improved SDS-alkaline lysis method to lyse the bacteria, the crude extract is selectively combined with a unique endotoxin scavenger and centrifuged to remove endotoxins, and then The silicon matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through the rinsing solution, and finally elution with low salt and high pH The buffer eluted purified plasmid DNA from the silicon matrix membrane. The silicon matrix membrane in the centrifugal adsorption column all adopts imported special adsorption membrane, the adsorption amount difference between the column and the column is very small, and the repeatability is good. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It can quickly extract 0.2-1.5 mg of pure high-copy plasmid DNA with an extraction rate of 80-90%. The unique process formula removes endotoxin, the content of endotoxin is very low (< 0.1 EU/μg DNA), and the cell transfection effect is excellent. It can also be directly used in various molecular biology experiments such as enzyme digestion, PCR, in vitro transcription, transformation, sequencing, and so on.   High Yield; Compatible with the extraction of plasmids of different lengths; The content of endotoxin is extremely low, and subsequent transfection level experiments can be done; Good stability   RNase A should be stored at -30 ~ -15℃; The endotoxin scavenger can be stored at 2-8℃ for one month, please store at -30 ~ -15℃ for long-term storage; The other components of the kit are stored at room temperature (15 ~ 25℃). 
116.00
Article number:
DC202
Number of views:
5085
Keywords:
endofree
the
and
is
of
in
endotoxin
dna
at
be
96.00
Article number:
DC103
This kit is suitable for extracting genomic DNA from various sources of bacteria (Gram-negative bacteria, Gram-positive bacteria). The kit uses silica gel membrane purification technology. There is no need to use toxic reagents such as phenol and chloroform during the extraction process, and no time-consuming alcohol precipitation, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.   Simple and fast; Wide range of applications; High yield and good integrity; High purity   RNase A and Proteinase K dry powder should be stored at -30~-15℃; Store other components at room temperature (15-25℃)    
96.00
Article number:
DC103
Number of views:
2976
Keywords:
75.00
Article number:
DC104
This kit is suitable for extracting genomic DNA from fresh and dried common plant samples or polysaccharide polyphenol plant samples. The kit uses silica gel membrane purification technology and a new and unique solution system. There is no need to use toxic reagents such as phenol, chloroform, or time-consuming alcohol precipitation during the extraction process, which can maximize the removal of impurity proteins and other organic compounds in plant cells. The extracted genomic DNA has high purity and stable quality, and can be used in molecular biology experiments such as PCR, enzyme digestion and hybridization.   Simple and fast; Wide range of applications; High yield and good integrity; High purity   RNase A is stored at -30~-15℃; Store other components at room temperature (15-25℃)
75.00
Article number:
DC104
Number of views:
2988
Keywords:
mini
and
the
high
is
plant
dna
in
as
at
13.00
Article number:
MD104-01/02
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright Band; the DNA concentration of all other bands is 50 ng/5 μl.   Good stability: it can be stored at room temperature for 6 months, all strips are clear and dense; Easy to operate: a ready-to-use product, you can directly take an appropriate amount of this product for electrophoresis when running the gel.    Store at -20°C; store at 4°C after thawing to avoid repeated freezing and thawing
13.00
Article number:
MD104-01/02
Number of views:
4203
Keywords:
10.90
Article number:
MD103-01/02
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright Band; the DNA concentration of all other bands is 50 ng/5 μl.   Good stability: it can be stored at room temperature for 6 months, all strips are clear and dense; Easy to operate: a ready-to-use product, you can directly take an appropriate amount of this product for electrophoresis when running the gel.    Store at -20°C; store at 4°C after thawing to avoid repeated freezing and thawing
10.90
Article number:
MD103-01/02
Number of views:
5023
Keywords:
8.70
Article number:
MD102-01/02
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, showing bright bands ; The DNA concentration of all other bands is 50 ng/5 μl.   Good stability: it can be stored at room temperature for 6 months, all strips are clear and dense; Easy to operate: a ready-to-use product, you can directly take an appropriate amount of this product for electrophoresis when running the gel.    Store at -20°C; store at 4°C after thawing to avoid repeated freezing and thawing
8.70
Article number:
MD102-01/02
Number of views:
7969
Keywords:
dna
the
and
in
is
of
bands
bp
marker
48.00
Article number:
P312-01/02/03
Vazyme LAmp® DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and a protein containing 3’→5’ exonuclease activity (proofreading activity). The fidelity is Taq DNA 6 times that of Polymerase. With a specially optimized buffer system, Vazyme LAmp® DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. This product contains Vazyme LAmp® DNAPolymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves throughput and results reproducibility. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. This product also provides a version containing electrophoresis buffer and dye, which can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).    Efficient amplification of genome and cDNA fragments >20 kb; Very high amplification sensitivity and strong tolerance to low purity templates; Broad GC adaptability; Provide ready-to-use premix to minimize experimental steps   Store at -20°C
48.00
Article number:
P312-01/02/03
Number of views:
3789
Keywords:
dye
and
the
to
is
of
dna
amplification
which
can
330.00
Article number:
P311-01/02/03
2×VazymeLamp®MasterMix  长距离PCR的明灯  高效扩增>20kb的基因组、cDNA片段极高的扩增灵敏度,对于低纯度模板具有很强的耐受度广泛的GC适应性 提供即用型的预混液,最大程度地减少实验步骤   图一极高的扩增性能以10ng不同种属的基因组DNA为模板,用VazymeLAmp® DNAPolymerase扩增长度为3.6-21.0kb的目的片段。 M.DL15000Ma
330.00
Article number:
P311-01/02/03
Number of views:
2718
Keywords:
P311-01/02/03
长距离PCR的明灯
长距离PCR的明灯
28.30
Article number:
P302-d1/d2
Vazyme LAmp® DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and a protein containing 3’→5’ exonuclease activity (proofreading activity). The fidelity is Taq DNA 6 times that of Polymerase. With a specially optimized buffer system, Vazyme LAmp® DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113). The attached PCR Enhancer can help the amplification of high GC content fragments.    Efficient amplification of genome and cDNA fragments >20 kb; Very high amplification sensitivity and strong tolerance to low purity templates; Broad GC adaptability; Provide ready-to-use premix to minimize experimental steps   Store at -20°C
28.30
Article number:
P302-d1/d2
Number of views:
2013
Keywords:
mg2+
the
and
of
amplification
dna
is
high
polymerase
for
28.30
Article number:
P301-d1/d2
Vazyme LAmp® DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and a protein containing 3’→5’ exonuclease activity (proofreading activity). The fidelity is Taq DNA 6 times that of Polymerase. With a specially optimized buffer system, Vazyme LAmp® DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113). The attached PCR Enhancer can help the amplification of high GC content fragments.    Efficient amplification of genome and cDNA fragments >20 kb; Very high amplification sensitivity and strong tolerance to low purity templates; Broad GC adaptability; Provide ready-to-use premix to minimize experimental steps   Store at -20°C
28.30
Article number:
P301-d1/d2
Number of views:
2019
Keywords:
mg2+
the
and
of
amplification
dna
is
high
polymerase
for
26.00
Article number:
P301-01/02
Vazyme LAmp® DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and a protein containing 3’→5’ exonuclease activity (proofreading activity). The fidelity is Taq DNA 6 times that of Polymerase. With a specially optimized buffer system, Vazyme LAmp® DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113). The attached PCR Enhancer can help the amplification of high GC content fragments.    Efficient amplification of genome and cDNA fragments >20 kb; Very high amplification sensitivity and strong tolerance to low purity templates; Broad GC adaptability; Provide ready-to-use premix to minimize experimental steps   Store at -20°C
26.00
Article number:
P301-01/02
Number of views:
2301
Keywords:

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